氮钾浓度对烤烟苗期生长特性的影响

探究氮钾浓度对烟苗生理生化特性的影响,为烟叶苗期氮钾营养平衡提供参考依据.采用水培法,利用基础Hoagland营养液,设置5个不同氮钾浓度处理,分析处理间烟苗的生理生化指标差异.结果表明,氮浓度对烟苗干物质、叶绿素的积累有显著影响,在相同钾浓度下,随着氮浓度的增加,烟苗干物质、叶绿素含量、根系活力显著增加.不同氮水平下,低钾处理间的根系MDA含量、叶片GS活性差异显著.K+对烟苗叶绿素的合成、根系活力、GS活性有一定的促进作用.高氮水平下,高浓度K+会使根系活力、叶片可溶性蛋白含量下降.     

Impact of grassland contract policy on soil organic carbon losses from alpine grassland on the Qinghai–Tibetan Plateau

Carbon storage in the soils on the Qinghai–Tibetan Plateau plays a very important role in the global carbon budget. In the 1990s, a policy of contracting collective grasslands to smaller units was implemented, resulting in a change from the traditional collective grassland management to two new management patterns: a multi‐household management pattern (MMP: grassland shared by several households without enclosures) and a single‐household management pattern (SMP: grassland enclosed and used by only one household). In 2016, 50 MMP and 54 SMP winter pastures on the Qinghai–Tibetan Plateau were sampled to assess the differences in soil organic carbon (SOC) between the two management patterns. Results showed that average SOC was significantly greater under MMP than under SMP, with an estimated 0.41 Mg C/ha/yr lost due to SMP following the new grassland contract. Based on the government's grassland policy, four grassland utilization scenarios were developed for both summer and winter pastures. We found that if the grassland were managed under SMP, likely C losses ranged between 0.31 × 10⁷ and 6.15 × 10⁷ Mg C/yr across the Qinghai–Tibetan Plateau relative to MMP, which more closely resembles pre‐1990s grassland management. Previous estimates of C losses have only considered land use change (with cover change) and ignored the impacts driven by land management pattern changes (without cover change). The new data suggest that C losses from the Qinghai–Tibetan Plateau are greater than previously estimated, and therefore that the grassland contract policy should be reviewed and SMP households should be encouraged to reunite into the MMP. These findings have potential implications for land management strategies not only on the Qinghai–Tibetan Plateau but also other grazing regions globally where such practices may exist.     

Identification and quantification of anthocyanins in different coloured cultivars of ornamental kale (Brassica oleracea L. var. acephala DC)

Anthocyanins are responsible for the colour of many fruits, vegetables, flowers, and coloured-leaved trees. Ornamental kale (Brassica oleracea L. var. acephala DC) is widely cultivated for its colourful inner leaves. To investigate the relationship between the degree of colouration and anthocyanin distribution, content, and composition in ornamental kale, the authors studied the pigment characteristics of five cultivars with different coloured leaves (white, pink, red, purple, and purple-black). Microscopy observation, spectrophotometer, and high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of fresh inner leaves revealed that pink, red, and purple colourations were associated with high levels of anthocyanin, while purple-black was the result of the combination of anthocyanins and chlorophyll. In the coloured cultivars, anthocyanins were abundant mainly in the first and second cell layers below the epidermis in both the hypocotyls and inner leaves. No anthocyanin was found in the white-leaved phenotype cultivar. Anthocyanin content increased as leaf colour deepened from pink, red, to purple cultivars, which had little chlorophyll and carotenoid. The authors identified eight anthocyanins in the four coloured cultivars, including one non-acylated, four monoacylated, and three diacylated cyanidin glycosides. Cyanidin-3-(sinapoyl)(feruloyl)-diglucoside-5-glucoside was the most abundant anthocyanin in the four coloured cultivars followed by cyanidin-3-(sinapoyl)-diglucoside-5-glucoside. The analysis of anthocyanin accumulation characterisation provides important information on evaluating colouration patterns in coloured plants, and will be helpful for breeding desired leaf colours in ornamental kale.     

日光温室新型装配式骨架节点设计与有限元分析

为实现日光温室骨架的装配化,把控安装质量,提高温室骨架受力性能,设计完成了日光温室新型装配式骨架及装配式节点,以期为装配式日光温室提供新的研究思路.利用ANSYS Workbench软件建模辅助分析,比选外套管和内插管2种装配方式,在逐级均布荷载作用下主管和套管所受应力、剪力及弯矩情况;用Workbench软件分析装配式骨架在最不利荷载作用下轴向应力、剪力、弯矩及变形情况.结果 表明:骨架在外套管的装配方式下,主管及套管自身所受的轴向应力和弯矩更小,安全性更高.用外套管装配的三段桁架在最不利荷载组合作用下,最大轴向应力17.35MPa,最大变形量为0.26 mm,套管最大轴向应力5.0 MPa,最大剪力2345.3 N,最大弯矩为41.3N·m.该日光温室新型装配式骨架及装配式节点安全、稳定、变形量小,可推广使用.     

武夷岩茶种质资源遗传多样性与亲缘关系的SRAP分析

本研究利用SRAP-PCR反应体系,从88对SRAP引物组合中筛选出43对引物组合,对武夷岩茶主要的30份种质资源进行SRAP分析,共扩增出385条带,其中具有多态性的有236条带,占总数的61.30%,表明30份武夷岩茶种质呈现一定的遗传多样性。武夷岩茶种质资源间遗传距离变幅在0.31~0.98之间,平均为0.63,基于SRAP标记利用系统聚类法把武夷岩茶分为三大类,为武夷岩茶遗传研究、品种选育与资源保护提供参考。     

我国国家公园与自然保护地法律制度衔接研究

我国既有的各类自然保护地存在空间交叉重叠、权责模糊、多头管理等突出问题，成为制约我国生态文明建设的体制难题。建立以国家公园为主体的自然保护地体系，正是国家破解这一难题的制度举措。文中在介绍国家公园与自然保护地概念、分析我国自然保护地现有法律制度存在主要问题的基础上，提出了国家公园与自然保护地法律制度衔接的设想。在国家公园体制建设的过程中，理顺国家公园与既有自然保护地之间的关系，加强国家公园与自然保护地法律制度的衔接显得尤为重要。具体思路为:在宏观政策法律层面，从国家统一立法、提升立法协调性、扩大公众参与等方面提出国家公园体制法律建构的整体设想；在微观制度保障层面，吸纳保护原则、差异原则及衔接原则，建立国家统一自然保护地管理制度。建议以《国家公园法》的制定为契机，统筹考量制定符合我国国情的统领自然空间保护的基本法——《自然保护地法》。     

有机肥对土壤特性及农产品产量和品质影响研究进展

施用有机肥是农业生产的重要措施,对绿色农业发展起到积极作用,而且越来越广泛施用。本文介绍了目前有机肥基本概况,重点综述了有机肥对提高土壤肥力、提高作物产量和品质、促进养分利用以及对病虫害发生影响等方面研究的最新进展,分析了当前有机肥施用对耕地质量和农产品安全风险、施肥效率低、以及有机肥品种研发不足、相关质量标准滞后等问题,并对当前有机肥推广应用的形势和发展前景进行了分析和展望。     

正交设计优化青蒿SRAP-PCR反应体系及引物筛选

为了建立青蒿的SRAP最佳扩增体系,并筛选出SRAP多态性引物,本研究以青蒿叶片DNA为模板,采用正交试验设计,以Mg^2+、dNTP Mix、Taq DNA聚合酶、引物和DNA模板5种因素5个水平,对青蒿SRAP反应体系进行研究。结果表明,青蒿SRAP-PCR最佳反应体系为:引物0.6μmol/L、Mg^2+2.0 mmol/L、模板DNA 5.1 ng、Taq DNA聚合酶2.0 U、dNTPs 0.25 mmol/L,总体积为25μL。各因素对扩增反应均有不同影响,其中引物浓度的影响最大,dNTPs的影响最小。运用该体系对不同种质资源的青蒿进行验证,证明该体系稳定可靠,并在30个引物组合中筛选出了25对扩增条带清晰,多态性丰富的引物组合。这一结论为今后利用SRAP标记技术进行青蒿分子遗传学研究提供了科学依据。     

采后UV-C处理对树莓贮藏保鲜效果的影响

以"September"树莓果实为材料,采用0.43、2.15、4.3和6.45 kJ/m2的UV-C进行辐照处理,并在4℃下贮藏,通过测定贮藏期间果实腐烂指数、失重率、可溶性固形物含量、总酚含量和抗氧化能力,研究UV-C处理对树莓果实保鲜效果的影响。结果表明,UV-C处理能够显著抑制果实腐烂指数、失重率的上升,减缓果实可溶性固形物含量、总酚含量的下降,增强果实的抗氧化能力,维持果实较好的品质。在4个UV-C处理剂量中,4.3 kJ/m2UV-C处理组果实的腐烂指数、失重率最低,可溶性固形物含量和总酚含量最高,抗氧化能力最强。适宜剂量的UV-C处理(4.3 kJ/m2)能维持树莓果实采后品质,增强抗性,延缓衰老。     

汽车变速器齿轮修形仿真分析

汽车变速器的关键部件齿轮因其加工和装配质量的影响,工作时容易引起振动噪声大及承载能力降低等问题,而齿轮修形是解决这些问题的有效方法,且成本较低。针对某款汽车变速器,利用Romax对齿轮进行修形分析。结果表明,修形后减小了齿轮的传动误差和最大接触应力,齿面载荷分布更加合理。有效改善了齿轮的传动性能,增加了齿轮寿命。